tailieunhanh - Báo cáo y học: "Thymic function and T cell parameters in a natural human experimental model of seasonal infectious diseases and nutritional burden"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Thymic function and T cell parameters in a natural human experimental model of seasonal infectious diseases and nutritional burden | Ngom et al. Journal of Biomedical Science 2011 18 41 http content 18 1 41 The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Thymic function and T cell parameters in a natural human experimental model of seasonal infectious diseases and nutritional burden Pa T Ngom1 Juan Solon1 Sophie E Moore1 Gareth Morgan1 Andrew M Prentice1 2 and Richard Aspinall3 Abstract Background The study exploits a natural human experimental model of subsistence farmers experiencing chronic and seasonally modified food shortages and infectious burden. Two seasons existed one of increased deprivation and infections Jul-Dec another of abundance and low infections Jan-Jun referred to as the hungry high infection and harvest low infection seasons respectively. Prior analysis showed a 10-fold excess in infectious disease associated mortality in young adults born in the hungry high infection versus harvest low infection season and reduced thymic output and T cell counts in infancy. Here we report findings on the role of early life stressors as contributors to the onset of T cell immunological defects in later life. Methods We hypothesised that season of birth effects on thymic function and T cell immunity would be detectable in young adults since Kaplan-Meier survival curves indicated this to be the time of greatest mortality divergence. T cell subset analyses by flow-cytometry sjTRECs TCRVp repertoire and telomere length by PCR were performed on samples from 60 males 18-23 y selected to represent births in the hungry high infection and harvest low infection Results Total lymphocyte counts were normal and did not differ by birth season. CD3 and CD4 but not CD8 counts were lower for those born during the hungry high infection season. CD8 telomere length also tended to be shorter. Overall CD8 TCRVp repertoire skewing was observed with public expressions and deletions .