tailieunhanh - Báo cáo y học: " Role of dopamine D2 receptors in ischemia/ reperfusion induced apoptosis of cultured neonatal rat cardiomyocytes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Role of dopamine D2 receptors in ischemia/ reperfusion induced apoptosis of cultured neonatal rat cardiomyocytes | Li et al. Journal of Biomedical Science 2011 18 18 http content 18 1 18 NSC The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Role of dopamine D2 receptors in ischemia reperfusion induced apoptosis of cultured neonatal rat cardiomyocytes 1 f f 3 4 1 1 1 1 Hong-zhu Li Jin Guo Jun Gao Li-ping Han Chun-ming Jiang Hong-xia Li Shu-zhi Bai Wei-hua Zhang Guang-wei Li1 5 Li-na Wang1 Hong Li1 Ya-jun Zhao1 Yan Lin5 Ye Tian1 Guang-dong Yang6 Rui Wang6 Ling-yun Wu7 Bao-feng Yang8 9 Chang-qing Xu1 9 Abstract Background Myocardial ischemia reperfusion injury is the major cause of morbidity and mortality for cardiovascular diseases. Dopamine D2 receptors are expressed in cardiac tissues. However the roles of dopamine D2 receptors in myocardial ischemia reperfusion injury and cardiomyocyte apoptosis are unclear. Here we investigated the effects of both dopamine D2 receptors agonist bromocriptine and antagonist haloperidol on apoptosis of cultured neonatal rat ventricular myocytes induced by ischemia reperfusion injury. Methods Myocardial ischemia reperfusion injury was simulated by incubating primarily cultured neonatal rat cardiomyocytes in ischemic hypoxic buffer solution for 2 h. Thereafter these cells were incubated for 24 h in normal culture medium. Results Treatment of the cardiomyocytes with 10 gM bromocriptine significantly decreased lactate dehydrogenase activity increased superoxide dismutase activity and decreased malondialdehyde content in the culture medium. Bromocriptine significantly inhibited the release of cytochrome c accumulation of Ca2 i and apoptosis induced by ischemia reperfusion injury. Bromocriptine also down-regulated the expression of caspase-3 and -9 Fas and Fas ligand and up-regulated Bcl-2 expression. In contrast haloperidol 10 gM had no significant effects on the apoptosis of cultured cardiomyocytes under the aforementioned

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