tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 115

Handbook of Microbiological Media, Fourth Edition part 115 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Methanospirillum SK Medium 1135 Preparation of Trace Minerals Solution Add nitrilotriacetic acid to of distilled deionized water. Adjust pH to with KOH. Add remaining components. Mix thoroughly. Add distilled deionized water to . Adjust pH to . Vitamin Solution Composition per liter Vitamin p-Aminobenzoic Lipoic Nicotinic Folic Vitamin Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. L-Cysteine Na2S Solution Composition per liter Preparation of L-Cysteine Na2S Solution Gently heat and bring of distilled deionized water to boiling. Add L-cysteine-HCl. Adjust pH to with 3N NaOH. Add Na2S-9H2O. Mix thoroughly. Gently heat and bring to boiling. Cool to room temperature while sparging with 100 N2. Bring volume to with distilled deionized water. Gently heat and bring to boiling. Cool to 60 C while sparging with 100 N2. Anaerobically distribute into serum bottles. Autoclave for 15 min at 15 psi pressure-121 C. FeSO4-6H2O Solution Composition per Preparation of FeSO4-6H2O Solution Add FeSO4-6H2O to dis-tilled deionized water and bring volume to . Mix thoroughly. Resazurin Solution Composition per Preparation of Resazurin Solution Add resazurin to distilled deionized water and bring volume to . Mix thoroughly. Preparation of Medium Prepare and dispense medium under 80 H2 20 CO2. Add components except Na2CO3 Na2CO3 solution and L-cysteine Na2S solution to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Gently heat and bring to boiling. Continue boiling for 3 min. Cool to 60 C while sparging with 80 H2 20 CO2. Add Na2CO3 and L-cysteine Na2S solution. Mix thoroughly. .