tailieunhanh - Serum cytokine profiles in healthy young and elderly population assessed using multiplexed bead-based immunoassays

Managed care prevalence in the market can also impact the climate in which the elderly seek care. The availability of managed care plans for the elderly could improve elderly access to and utilization of preventive care services, if the Medicare managed care plans fulfill their promise – more specifically, the management and coordination of care. A growing body of literature has found that Medicare beneficiaries in HMOs receive more preventive services and have better outcomes than their FFS counterparts. Rizzo [29] found that Medicare beneficiaries enrolled in HMOs received significantly and substantially higher preventive care services than beneficiaries in traditional FFS. Other research has found that managed care may improve access for the. | Kim et al. Journal of Translational Medicine 2011 9 113 http content 9 1 113 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Serum cytokine profiles in healthy young and elderly population assessed using multiplexed bead-based immunoassays Hyun Ok Kim1t Han-Soo KimH Jong-Chan Youn 2 Eui-Cheol Shin3 and Sungha Park2 Abstract Background Lipid metabolites and cytokines including chemokines and growth factors are the key regulators of immune cell function and differentiation and thus dysregulation of these regulators is associated with various human diseases. However previous studies demonstrating a positive correlation of cytokine levels with aging may have been influenced by various environmental factors and underlying diseases. Also data regarding cytokine profiling in the elderly are limited to a small subset of cytokines. Methods We compared the profiles of 22 cytokines including chemokines and growth factors in a case-controlled study group of a gender-matched healthy cohort of 55 patients over the age of 65 and 55 patients under the age of 45. Assessment of serum cytokine concentrations was performed using commercially-available multiplex bead-based sandwich immunoassays. Results Soluble CD40 ligand sCD40L and transforming growth factor alpha TGF-a levels were significantly higher in the elderly patients whereas granulocyte colony-stimulating factor G-CSF granulocyte-monocyte colony-stimulating factor GM-CSF and monocyte chemoattractant protein-1 MCP-1 levels were significantly lower in the elderly patients. The partial correlation analysis demonstrating the correlation between cytokine levels when controlled for gender systolic blood pressure total cholesterol HDL cholesterol triglyceride and serum creatinine levels further demonstrated that G-CSF GM-CSF and MCP-1 had significant negative correlations with age whereas sCD40L and TGF-a had significant positive correlations. Conclusions Future studies will focus on examining