tailieunhanh - Báo cáo y học: "Stable gene transfer of CCR5 and CXCR4 siRNAs by sleeping beauty transposon system to confer HIV-1 resistance"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Stable gene transfer of CCR5 and CXCR4 siRNAs by sleeping beauty transposon system to confer HIV-1 resistance. | AIDS Research and Therapy BioMed Central Research Stable gene transfer of CCR5 and CXCR4 siRNAs by sleeping beauty transposon system to confer HIV-1 resistance Mayur Tamhane and Ramesh Akkina Address Dept. Microbiology Immunology and Pathology Colorado State University Fort Collins Colorado 80523 USA Email MayurTamhane - mayur@ Ramesh Akkina - akkina@ Corresponding author Open Access Published 30 July 2008 Received 25 March 2008 AIDS Research and Therapy 2008 5 16 doi 1742-6405-5-16 Accepted 30 July 2008 This article is available from http content 5 1 16 2008 Tamhane and Akkina licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Thus far gene therapy strategies for HIV AIDS have used either conventional retroviral vectors or lentiviral vectors for gene transfer. Although highly efficient their use poses a certain degree of risk in terms of viral mediated oncogenesis. Sleeping Beauty SB transposon system offers a non-viral method of gene transfer to avoid this possible risk. With respect to conferring HIV resistance stable knock down of HIV-1 coreceptors CCR5 and CXCR4 by the use of lentiviral vector delivered siRNAs has proved to be a promising strategy to protect cells from HIV-1 infection. In the current studies our aim is to evaluate the utility of SB system for stable gene transfer of CCR5 and CXCR4 siRNA genes to derive HIV resistant cells as a first step towards using this system for gene therapy. Results Two well characterized siRNAs against the HIV-1 coreceptors CCR5 and CXCR4 were chosen based on their previous efficacy for the SB transposon gene delivery. The siRNA transgenes were incorporated individually into a modified SB transfer plasmid