tailieunhanh - Báo cáo y học: " Substitution of the Rev-response element in an HIV-1-based gene delivery system with that of SIVmac239 allows efficient delivery of Rev M10 into T-lymphocytes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Substitution of the Rev-response element in an HIV-1-based gene delivery system with that of SIVmac239 allows efficient delivery of Rev M10 into T-lymphocytes. | AIDS Research and Therapy BioMed Central Research Substitution of the Rev-response element in an HIV-l-based gene delivery system with that of SIVmac239 allows efficient delivery of Rev M10 into T-lymphocytes Narasimhachar Srinivasakumar Open Access Address Division of Hematology Oncology Department of Medicine Vanderbilt University Nashville Tennessee USA Email Narasimhachar Srinivasakumar - Published 5 June 2008 Received 7 April 2008 AIDS Research and Therapy 2008 5 11 doi 1742-6405-5-11 Accepted 5 June 2008 This article is available from http content 5 1 1 1 2008 Srinivasakumar licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Human immunodeficiency virus type 1 HIV-1 -based gene delivery systems are popular due to their superior efficiency of transduction of primary cells. However these systems cannot be readily used for delivery of anti-HIV-1 genes that target constituents of the packaging system itself due to inimical effects on vector titer. Here we describe HIV-1-based packaging systems containing the Rev-response element RRE of simian immunodeficiency virus SIV in place of the HIV-1 RRE. The SIV RRE-containing packaging systems were used to deliver the antiRev gene Rev M10 into HIV-1 susceptible target cells. Results An HIV-1 based packaging system was created using either a 272- or 1045-nucleotide long RRE derived from the molecular clone SIVmac239. The 1045-nucleotide SIV RRE-containing HIV-1 packaging system provided titers comparable to that of the HIV-1 RRE-based one. Moreover despite the use of HIV-1 Rev for production of vector stocks this packaging system was found to be relatively refractory to the inhibitory effects of Rev M10.