tailieunhanh - Báo cáo y học: "Instability of retroviral vectors with HIV-1-specific RT aptamers due to cryptic splice sites in the U6 promoter"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Instability of retroviral vectors with HIV-1-specific RT aptamers due to cryptic splice sites in the U6 promoter. | AIDS Research and Therapy BioMed Central Research Open Access Instability of retroviral vectors with HIV-1-specific RT aptamers due to cryptic splice sites in the U6 promoter Stephen E Braun 1 Xuanling Shi2 Gang Qiu1 Fay Eng Wong1 Pheroze J Joshi2 3 Vinayaka R Prasad2 and R Paul Johnson1 4 Address 1Division of Immunology New England Primate Research Center Harvard Medical School One Pine Hill Drive Southborough MA 01772 USA 2Albert Einstein College of Medicine Bronx 1300 Morris Park Avenue Room GB 401 Bronx NY 10461 USA 3J. David Gladstone Institute of Virology and Immunology University of California at San Francisco 1650 Owens Street San Francisco CA 94158 USA and 4Partners AIDS Research Unit and Infectious Disease Unit Massachusetts General Hospital Boston MA 02115 USA Email Stephen E Braun - stephen_braun@ Xuanling Shi - xushi@ Gang Qiu - gang_qiu@ Fay Eng Wong - fay_eng@ Pheroze J Joshi - pjoshi@ Vinayaka R Prasad - prasad@ R Paul Johnson - paul_johnson@ Corresponding author Published 17 October 2007 Received 16 January 2006 AIDS Research and Therapy 2007 4 24 doi l742-6405-4-24 Accepted 17 October 2007 This article is available from http contentM 1 24 2007 Braun et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Internal polymerase III promoters in retroviral vectors have been used extensively to express short RNA sequences such as ribozymes RNA aptamers or short interfering RNA inhibitors in various positions and orientations. However the stability of these promoters in the reverse orientation has not been rigorously evaluated. Results A series of retroviral .