tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 110

Handbook of Microbiological Media, Fourth Edition part 110 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Methanobacterium II Medium 1085 use inject for each medium sterile NaHCO3 solution sterile Na2S-9H2O solution and sterile methanol solution. The pH should be . Use For the cultivation of Methanosarcina barkeri DSM 10131 and Methanosarcina mazei Methanococcus mazei Methanosarcina frisia DSM 10132. Methanobacterium II Medium DSMZ Medium 825 Composition per Yeast Sodium NaHCO3 Na-formate Trace elements Vitamin Na2S-9H2O pH at 25 C Na-Formate Solution Composition per Na-formate . Preparation of Na-Formate Solution Add Na-formate to dis-tilled deionized water and bring volume to . Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Trace Elements Solution Composition per liter Nitrilotriacetic KAl SO4 Preparation of Trace Elements Solution Add nitrilotriacetic acid to of distilled deionized water. Dissolve by adjusting pH to with KOH. Add remaining components. Add distilled deionized water to . Mix thoroughly. Vitamin Solution Composition per liter Nicotinic p-Aminobenzoic Lipoic Folic Vitamin Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Sparge with 80 H2 20 CO2. Filter sterilize. Na2S-9H2O Solution Composition per Preparation of Na2S-9H2O Solution Add Na2S-9H2O to dis-tilled .