tailieunhanh - Báo cáo y học: "Protein kinases modulate store-operated channels in pulmonary artery smooth muscle cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Protein kinases modulate store-operated channels in pulmonary artery smooth muscle cells | Chen et al. Journal of Biomedical Science 2011 18 2 http content 18 1 2 NSC The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Protein kinases modulate store-operated channels in pulmonary artery smooth muscle cells I-Shan Chen1 Zen-Kong Dai2 Donald G Welsh3 Ing-Jun Chen1 Bin-Nan Wu1 Abstract Background This study investigates whether protein kinase G PKG protein kinase A PKA and protein kinase C PKC are involved in the regulatory mechanisms of store-operated channel SOC in pulmonary arteries. Methods Pulmonary artery smooth muscle cells PASMCs were enzymatically dissociated from rat intralobar pulmonary arteries. Whole cell cell-attached and inside-out patch-clamp electrophysiology were used to monitor SOCs in isolated PASMCs. Results Initially the Ca2 -ATPase inhibitor cyclopiazonic acid CPA 10 gM initiated a whole cell current that was reduced by the SOC blocker SKF-96365 10 gM . Subsequent work using both cell-attached and whole cell configurations revealed that the PKG and PKA inhibitors KT5823 3 gM and H-89 10 gM also stimulated SOC activity this augmentation was attenuated by the SOC blockers SKF-96365 10 gM and Ni2 mM . Finally using the inside-out configuration the PKC activator phorbol 12-myristate 13-acetate PMA 10 gM was confirmed to modestly stimulate SOC activity although this augmentation appeared to be more substantial following the application of 10 gM inositol 1 4 5-triphosphate Ins 1 4 5 P3 . Conclusions SOC activity in PASMCs was stimulated by the inhibition of PKG and PKA and the activation of PKC. Our findings suggest that the SOC could be a substrate of these protein kinases which therefore would regulate the intracellular concentration of calcium and pulmonary arteriopathy via SOC. Background Intracellular calcium Ca2 i is an important second messenger responsible for many physiological functions including .