tailieunhanh - Báo cáo y học: "Regional characterization of energy metabolism in the brain of normal and MPTP-intoxicated mice using new markers of glucose and phosphate transport"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Regional characterization of energy metabolism in the brain of normal and MPTP-intoxicated mice using new markers of glucose and phosphate transport | Lagrue et al. Journal of Biomedical Science 2010 17 91 http content 17 1 91 BIN sc The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Regional characterization of energy metabolism in the brain of normal and MPTP-intoxicated mice using new markers of glucose and phosphate transport E m m I I I I I z i ri I 1 2 3 I_I ỉ rz I 11 1 A p. 4 5 61 It T I m ì4 5 7 I n I z- . I 11 . s rn i4 5O zk i D z s r d 1 2 Emmanuelle Lagrue niioyuKi Abe MauaKasira Lavanya Jawida lounami Sylvie Bodard Sylvie Chalon1 2 Jean-Luc Battini4 5 Marc Sitbon4 5 Pierre Castelnau1 2 3 Abstract The gibbon ape leukemia virus GALV the amphotropic murine leukemia virus AMLV and the human T-cell leukemia virus HTLV are retroviruses that specifically bind nutrient transporters with their envelope glycoproteins Env when entering host cells. Here we used tagged ligands derived from GALV AMLV and HTLV Env to monitor the distribution of their cognate receptors the inorganic phosphate transporters PiT1 and PiT2 and the glucose transporter GLUT1 respectively in basal conditions and after acute energy deficiency. For this purpose we monitored changes in the distribution of PiT1 PiT2 and GLUT1 in the cerebellum the frontal cortex the corpus callosum the striatum and the substantia nigra SN of C57 BL6 mice after administration of 1-methyl-4-phenyl-1 2 3 6 tetrahydropyridinium MPTP a mitochondrial complex I inhibitor which induces neuronal degeneration in the striato-nigral network. The PiT1 ligand stained oligodendrocytes in the corpus callosum and showed a reticular pattern in the SN. The PiT2 ligand stained particularly the cerebellar Purkinje cells while GLUT1 labelling was mainly observed throughout the cortex basal ganglia and cerebellar gray matter. Interestingly unlike GLUT1 and PiT2 distributions which did not appear to be modified by MPTP intoxication PiT1 immunostaining seemed to