tailieunhanh - Báo cáo y học: "Comparison of capillary based microflurometric assay for CD4+ T cell count estimation with dual platform Flow cytometr"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Comparison of capillary based microflurometric assay for CD4+ T cell count estimation with dual platform Flow cytometry. | AIDS Research and Therapy BioMed Central Research Comparison of capillary based microflurometric assay for CD4 T cell count estimation with dual platform Flow cytometry Madhuri RThakar B Kishore Kumar Bharati A Mahajan Sanjay M Mehendale and Ramesh S Paranjape Open Access Address National AIDS Research Institute Bhosari Pune 411026 India Email Madhuri RThakar - mthakar@ B Kishore Kumar - kishorekumar_b@ Bharati A Mahajan - mahajanbharati@ Sanjay M Mehendale - smehendale@ Ramesh S Paranjape - rparanjape@ Corresponding author Published 16 October 2006 Received 26 June 2006 AIDS Research and Therapy 2006 3 26 doi l742-6405-3-26 Accepted 16 October 2006 This article is available from http content 3 1 26 2006 Thakar et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The CD4 T cell count estimation is an important monitoring tool for HIV disease progression and efficacy of anti-retroviral treatment ART . Due to availability of ART at low cost in developing countries quest for reliable cost effective alternative methods for CD4 T cell count estimation has gained importance. A simple capillary-based microflurometric assay EasyCD4 System Guava Technology was compared with the conventional flow cytometric assay for estimation of CD4 T cell counts in 79 HIV infected individuals. CD4 T cell count estimation by both the assays showed strong correlation r p 95 CI to . The Bland Altman plot analysis showed that the limits of variation were within agreeable limits of 2SD -161 to 129 cells mm3 . The Easy CD4 assay showed l00 sensitivity for estimating the CD4 T cell counts 200 cells mm3 and 350 cells mm3 and 97 .