tailieunhanh - Báo cáo y học: "Improving therapeutic HPV peptide-based vaccine potency by enhancing CD4+ T help and dendritic cell activation"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Improving therapeutic HPV peptide-based vaccine potency by enhancing CD4+ T help and dendritic cell activation | Wu et al. Journal of Biomedical Science 2010 17 88 http content 17 1 88 The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Improving therapeutic HPV peptide-based vaccine potency by enhancing CD4 T help and dendritic cell activation Chao-Yi Wu1 Archana Monie1 Xiaowu Pang5 Chien-Fu Hung1 4 T-C Wu1 2 3 4 Abstract Background Effective vaccination against human papillomavirus HPV represents an opportunity to control cervical cancer. Peptide-based vaccines targeting HPV E6 and or E7 antigens while safe will most likely require additional strategies to enhance the vaccine potency. Methods We tested the HPV-16 E7 peptide-based vaccine in combination with a strategy to enhance CD4 T help using a Pan HLA-DR epitope PADRE peptide and a strategy to enhance dendritic cell activation using the toll-like receptor 3 ligand poly I C . Results We observed that mice vaccinated with E7 peptide-based vaccine in combination with PADRE peptide and poly I C generated better E7-specific CD8 T cell immune responses as well as significantly improved therapeutic anti-tumor effects against TC-1 tumors compared to E7 peptide-based vaccine with either PADRE peptide or poly I C alone. Furthermore we found that intratumoral vaccination with the E7 peptide in conjunction with PADRE peptide and poly I C generates a significantly higher frequency of E7-specific CD8 T cells as well as better survival compared to subcutaneous vaccination with the same regimen in treated mice. Conclusions The combination of PADRE peptide and poly I C with antigenic peptide is capable of generating potent antigen-specific CD8 T cell immune responses and antitumor effects in vaccinated mice. Our study has significant clinical implications for peptide-based vaccination. Introduction Cervical cancer is the 2nd leading cause of cancer deaths in women worldwide. The primary etiological factor in