tailieunhanh - Báo cáo y học: "Precise pattern of recombination in serotonergic and hypothalamic neurons in a Pdx1-cre transgenic mouse line"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Precise pattern of recombination in serotonergic and hypothalamic neurons in a Pdx1-cre transgenic mouse line | Honig et al. Journal of Biomedical Science 2010 17 82 http content 17 1 82 The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Precise pattern of recombination in serotonergic and hypothalamic neurons in a Pdx1-cre transgenic mouse line Gerard Honig1 5 7 Angela Liou2 4 Miles Berger3 5 6 Michael S German4 Laurence H Tecott5 Abstract Background Multicellular organisms are characterized by a remarkable diversity of morphologically distinct and functionally specialized cell types. Transgenic techniques for the manipulation of gene expression in specific cellular populations are highly useful for elucidating the development and function of these cellular populations. Given notable similarities in developmental gene expression between pancreatic p-cells and serotonergic neurons we examined the pattern of Cre-mediated recombination in the nervous system of a widely used mouse line Pdx1-cre formal designation Tg Ipf1-cre in which the expression of Cre recombinase is driven by regulatory elements upstream of the pdxl pancreatic-duodenal homeobox 1 gene. Methods Single hemizygous transgenic mice of the pdx1-creCre 0 genotype were bred to single hemizygous transgenic reporter mice Z EG and rosa26R lines . Recombination pattern was examined in offspring using wholemount and sectioned histological preparations at and adult developmental stages. Results In addition to the previously reported pancreatic recombination recombination in the developing nervous system and inner ear formation was observed. In the central nervous system we observed a highly specific pattern of recombination in neuronal progenitors in the ventral brainstem and diencephalon. In the rostral brainstem r1-r2 recombination occurred in newborn serotonergic neurons. In the caudal brainstem recombination occurred in non-serotonergic cells. In the adult .