tailieunhanh - Báo cáo y học: " Over-expression of HO-1 on mesenchymal stem cells promotes angiogenesis and improves myocardial function in infarcted myocardium"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Over-expression of HO-1 on mesenchymal stem cells promotes angiogenesis and improves myocardial function in infarcted myocardium | Zeng et al. Journal of Biomedical Science 2010 17 80 http content 17 1 80 The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Over-expression of HO-1 on mesenchymal stem cells promotes angiogenesis and improves myocardial function in infarcted myocardium Bin Zeng1 Guosheng Lin1 Xiaofeng Ren2 Yan Zhang1 Honglei Chen3 Abstract Heme oxygenase-1 HO-1 is a stress-inducible enzyme with diverse cytoprotective effects and reported to have an important role in angiogenesis recently. Here we investigated whether HO-1 transduced by mesenchymal stem cells MSCs can induce angiogenic effects in infarcted myocardium. HO-1 was transfected into cultured MSCs using an adenoviral vector. 1 X 106 Ad-HO-1-transfected MSCs HO-1-MSCs or Ad-Null-transfected MSCs Null-MSCs or PBS was respectively injected into rat hearts intramyocardially at 1 h post-myocardial infarction. The results showed that HO-1-MSCs were able to induce stable expression of HO-1 in vitro and in vivo. The capillary density and expression of angiogenic growth factors VEGF and FGF2 were significantly enhanced in HO-1-MSCs-treated hearts compared with Null-MSCs-treated and PBS-treated hearts. However the angiogenic effects of HO-1 were abolished by treating the animals with HO inhibitor zinc protoporphyrin. The myocardial apoptosis was marked reduced with significantly reduced fibrotic area in HO-1-MSCs-treated hearts Furthermore the cardiac function and remodeling were also significantly improved in HO-1-MSCs-treated hearts. Our current findings support the premise that HO-1 transduced by MSCs can induce angiogenic effects and improve heart function after acute myocardial infarction. Introduction Recent pre-clinical and clinical studies have demonstrated that mesenchymal stem cells MSCs transplantation can attenuate ventricular remodeling and augment cardiac function when implanted into the .