tailieunhanh - A potential protective effect of α-tocopherol on vascular complication in spinal cord reperfusion injury in rats

Paraplegia remains a potential complication of spinal cord ischemic reperfusion injury (IRI) in which oxidative stress induced cyclooxygenase activities may contribute to ischemic neuronal damage. Prolonged administration of vitamin E (α-TOL), as a potent biological antioxidant, may have a protective role in this oxidative inflammatory ischemic cascade to reduce the incidence of paraplegia. The present study was designed to evaluate the preventive value of α-TOL in IRI of spinal cord. Methods: For this study, 50 male Sprague-Dawley rats were used and divided into five experimental groups (n = 10): Control group (C); α-TOL control group (CE) which received. | Morsy et al. Journal of Biomedical Science 2010 17 55 http content 17 1 55 a NSC The cost of publication In Journal of Blomodlcal Science Is boms by tlM National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access A potential protective effect of a-tocopherol on vascular complication in spinal cord reperfusion injury in rats Mohamed D Morsy 1 Ossama A Mostafa2 and Waleed N Hassan3 Abstract Background Paraplegia remains a potential complication of spinal cord ischemic reperfusion injury IRI in which oxidative stress induced cyclooxygenase activities may contribute to ischemic neuronal damage. Prolonged administration of vitamin E a-TOL as a potent biological antioxidant may have a protective role in this oxidative inflammatory ischemic cascade to reduce the incidence of paraplegia. The present study was designed to evaluate the preventive value of a-TOL in IRI of spinal cord. Methods For this study 50 male Sprague-Dawley rats were used and divided into five experimental groups n 10 Control group C a-TOL control group CE which received intramuscular . a-TOL injections 600 mg kg Sham operated group S IRI rats were subjected to laparotomy and clamping of the aorta just above the bifurcation for 45 min then the clamp was released for 48 hrs for reperfusion and IRIE rats group received 600 mg kg of a-TOL . twice weekly for 6 weeks followed by induction of IRI similar to the IRI group. At the end of the experimental protocol motor sensory and placing stepping reflex evaluation was done. Plasma nitrite nitrate NOx was measured. Then animals spinal cord lumbar segments were harvested and homogenized for measurement of the levels of prostaglandin E2 PGE2 malondialdehyde MDA and advanced oxidation products AOPP while superoxide dismutase SOD and catalase CAT activity were evaluated. Results Induction of IRI in rats resulted in significant increases in plasma levels of nitrite nitrate p and spinal cord homogenate levels of .