tailieunhanh - Báo cáo y học: "Suberoylanilide hydroxamic acid induces apoptosis and sub-G1 arrest of 320 HSR colon cancer cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Suberoylanilide hydroxamic acid induces apoptosis and sub-G1 arrest of 320 HSR colon cancer cells | Sun et al. Journal of Biomedical Science 2010 17 76 http content 17 1 76 The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Suberoylanilide hydroxamic acid induces apoptosis and sub-G1 arrest of 320 HSR colon cancer cells Pei-Chang SunH Ching Tzao2t Ban-Hen Chen 3 Chen-Wei Liu3 Cheng-Ping Yu4 Jong-Shiaw Jin4 Abstract Background Histone deacetylases and histone acetyl transferases covalently modify histone proteins consequentially altering chromatin architecture and gene expression. Methods The effects of suberoylanilide hydroxamic acid a HDAC inhibitor on 320 HSR colon cells were assessed in 320 HSR colon cancer cells. Results Concentration and time-dependent inhibition of 320 HSR cell proliferation was observed. Treatment of 320 HSR cells with 5 gM SAHA for 72 h significantly inhibited their growth by 50 as compared to that of the control. Fluorescence-activated cell sorting analysis demonstrated significant inhibition of cell cycle progression sub-G1 arrest and induction of apoptosis upon various SAHA concentrations after 48 h. In addition the antiapoptosis proteins survivin and Bcl-xL were significantly inhibited by SAHA after 72 h of treatment. Immunocytochemistry analysis revealed that SAHA-resistant cells were positive for cyclin A 85 ki-67 100 p53 100 survivin 100 and p21 90 expression. Furthermore a significant increase cyclin A- Ki-67- p53- survivin- and p21-positive cells were noted in SAHA-resistant tumor cells. Conclusion Our results demonstrated for the first time in 320 HSR colon adenocarcinoma cells that SAHA might be considered as an adjuvant therapy for colon adenocarcinoma. Background Histone deacetylases HDAC and histone acetyltransferase have antagonistic actions on histones depending on the cell state 1 2 . Epigenetic regulation of gene expression has been the subject of growing interest and HDAC inhibitors HDACi .