tailieunhanh - Identification of baboon microRNAs expressed in liver and lymphocytes

MicroRNAs (miRNAs) are small noncoding RNAs (~22 nucleotides) that regulate gene expression by cleaving mRNAs or inhibiting translation. The baboon is a well-characterized cardiovascular disease model; however, no baboon miRNAs have been identified. Evidence indicates that the baboon and human genomes are highly conserved; based on this conservation, we hypothesized that comparative genomic methods could be used to identify baboon miRNAs. Methods: We employed an in silico comparative genomics approach and human miRNA arrays to identify baboon expressed miRNAs in liver (n = 6) and lymphocytes (n = 6). Expression profiles for selected miRNAs in multiple tissues were validated by. | Karere et al. Journal of Biomedical Science 2010 17 54 http content 17 1 54 a NSC Tha cost of publication In Journal of Blomodlcal Science Is boms by tlM National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Identification of baboon microRNAs expressed in liver and lymphocytes Genesio M Karere 1 Jeremy P Glenn1 John L VandeBerg1 2 and Laura A Cox 1 2 Abstract Background MicroRNAs miRNAs are small noncoding RNAs 22 nucleotides that regulate gene expression by cleaving mRNAs or inhibiting translation. The baboon is a well-characterized cardiovascular disease model however no baboon miRNAs have been identified. Evidence indicates that the baboon and human genomes are highly conserved based on this conservation we hypothesized that comparative genomic methods could be used to identify baboon miRNAs. Methods We employed an in silico comparative genomics approach and human miRNA arrays to identify baboon expressed miRNAs in liver n 6 and lymphocytes n 6 . Expression profiles for selected miRNAs in multiple tissues were validated by RT-PCR. Results We identified in silico 555 putative baboon pre-miRNAs of which 41 exhibited 100 identity and an additional 58 shared more than 90 sequence identity with human pre-miRNAs. Some of these miRNAs are primatespecific and are clustered in the baboon genome like human miRNA clusters. We detected expression of 494 miRNAs on the microarray and validated expression of selected miRNAs in baboon liver and lymphocytes by RT-PCR. We also observed miRNA expression in additional tissues relevant to dyslipidemia and atherosclerosis. Approximately half of the miRNAs expressed on the array were not predicted in silico suggesting that we have identified novel baboon miRNAs which could not be predicted using the current draft of the baboon genome. Conclusion We identified a subset of baboon miRNAs using a comparative genomic approach identified additional baboon miRNAs using a human array and showed

• Báo cáo khoa học
• statements and documents scientific reports
• medical reports
• medical knowledge
• medical research
• medical research
• health care
• Health careassociated infections
• Antimicrobial susceptibility testing
• Health care worker
• Tertiary care hospital
• Health care workers
• Staphylococcus aureus
• Methicillin resistance staphylococcus aureus
• Health care workers in a tertiary care center
• Prevalence of carriage
• Health care personnel
• Health care skills
• Phlebotomy for health care personnel
• History of Phlebotomy
• Regulatory agencies
• Infection control
• Blood circulation
• Coronary circulation
• Pulmonary circulation
• Specimen collection
• Equipment for specimen collection
• Laboratory requisitions
• Performing venipuncture
• Dermal puncture
• Patient positioning
• Specimen handling
• Specimen processing
• Blood cultures
• Special phlebotomy procedures
• Glucose testing
• Bleeding time procedure
• Phlebotomy continuing education
• Practicing phlebotomy
• Quality assurance in phlebotomy
• Service quality management on health care industry in Bangalore
• Health care industry in Bangalore
• Health care spending in America
• Larger share of GDP than
• Gross domestic produc
• Home Health Care
• Health Care Routing
• Simulated Annealing algorithm
• Staff vehicle traveling
• Health Care system
• Hepatitis B virus
• Anti HBs titre
• Hepatitis B vaccination
• Joint annual health review 2015
• Socio economic situation
• Strengthening primary health care
• Health coverage
• Grassroots health network
• BMC Pediatrics
• Immediate newborn care
• District health offices
• Regional health bureau
• Health facilities
• Economic management
• Summary of Economic dissertation
• Quality of health care services
• Health insurance at district hospitals
• Health care services
• Health financial burden
• Health care services of households
• Health expenditure
• Health insurance
• Basis health level
• Hospital fee
• Out of pocket
• Lecture Economics
• Demand analysis to health care
• Copayment rate
• Demand for health care
• Market equilibrium
• Summary of Doctoral thesis
• Public private partnership
• Providing health care services
• Application of PPP in the health care
• Public health
• Usual source of care
• Cardiovascular diseases
• Health care seeking
• CVD related health care
• Health information management
• Health care delivery systems
• Health information management professionals
• Health care settings
• Electronic health records
• The patient record
• Educational attainment levels
• Nontraditional health information resources
• Findings from the Canadian survey
• Experiences with primary health care
• Primary health care
• Mupirocin resistance
• Health care provider
• Nasal swab
• Health care providers with MRSA
• Nosocomial infections
• Bio medical waste
• Hand hygiene
• Health care associated infections
• geographical dimension
• time dimension
• mental illness
• health campaigns
• Health information technology
• Improve health
• care quality
• Prevent medical errors
• Reduce health care costs
• Increase administrative efficiencies
• Decrease paperwork
• affordable care