tailieunhanh - GT-repeat polymorphism in the heme oxygenase1 gene promoter and the risk of carotid atherosclerosis related to arsenic exposure

Arsenic is a strong stimulus of heme oxygenase (HO)-1 expression in experimental studies in response to oxidative stress caused by a stimulus. A functional GT-repeat polymorphism in the HO-1 gene promoter was inversely correlated to the development of coronary artery disease in diabetics and development of restenosis following angioplasty in patients. The role of this potential vascular protective factor in carotid atherosclerosis remains unclear. We previously reported a graded association of arsenic. | Wu et al. Journal of Biomedical Science 2010 17 70 http content 17 1 70 NSC The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access GT-repeat polymorphism in the heme oxygenase-1 gene promoter and the risk of carotid atherosclerosis related to arsenic exposure Meei-Maan Wu1 2 3 Hung-Yi Chiou 1 Te-Chang Lee4 Chi-Ling Chen5 Ling-I Hsu6 Yuan-Hung Wang7 Wen-Ling Huang1 Yi-Chen Hsieh1 Tse-Yen Yang6 Cheng-Yeh Lee6 Ping-Keung Yip8 Chih-Hao Wang9 Yu-Mei Hsueh1 Chien-Jen Chen6 Abstract Background Arsenic is a strong stimulus of heme oxygenase HO -1 expression in experimental studies in response to oxidative stress caused by a stimulus. A functional GT-repeat polymorphism in the HO-1 gene promoter was inversely correlated to the development of coronary artery disease in diabetics and development of restenosis following angioplasty in patients. The role of this potential vascular protective factor in carotid atherosclerosis remains unclear. We previously reported a graded association of arsenic exposure in drinking water with an increased risk of carotid atherosclerosis. In this study we investigated the relationship between HO-1 genetic polymorphism and the risk of atherosclerosis related to arsenic. Methods Three-hundred and sixty-seven participants with an indication of carotid atherosclerosis and an additional 420 participants without the indication which served as the controls from two arsenic exposure areas in Taiwan a low arsenic-exposed Lanyang cohort and a high arsenic-exposed LMN cohort were studied. Carotid atherosclerosis was evaluated using a duplex ultrasonographic assessment of the extracranial carotid arteries. Allelic variants of GT n repeats in the 5 -flanking region of the HO-1 gene were identified and grouped into a short S allele 27 repeats and long L allele 27 repeats . The association of atherosclerosis and the HO-1 genetic variants was