tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 105

Handbook of Microbiological Media, Fourth Edition part 105 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | M-CP Agar Base 1035 cally add of sterile egg yolk emulsion 50 . Mix thoroughly. Pour into sterile Petri dishes in volumes. Use For the cultivation of Clostridium botulinum. McClung-Toabe Egg Yolk Agar CDC Modified CDC Modified McClung-Toabe Egg Yolk Agar Composition per liter Pancreatic digest of Yeast Egg yolk MgSO4 5 solution . pH at 25 C Egg Yolk Emulsion Composition Chicken egg Whole chicken Preparation of Egg Yolk Emulsion Soak eggs with 1 100 dilution of saturated mercuric chloride solution for 1 min. Crack eggs. Separate yolks from whites for 11 eggs. Mix egg yolks with 1 chicken egg. Preparation of Medium Add components except egg yolk emulsion to distilled deionized water and bring volume to . Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 60 C. Aseptically add of sterile egg yolk emulsion. Mix thoroughly. Pour into sterile Petri dishes in volumes. Use For the isolation cultivation and differentiation of anaerobic bacteria from foods. Bacteria that produce lecithinase appear as colonies surrounded by an insoluble opaque precipitate. Bacteria that produce lipase activity appear as colonies with a sheen or pearly surface. Bacteria that possess proteolytic activity appear as colonies surrounded by a clear zone. McClung-Toabe Egg Yolk Agar CDC Modified CDC Modified McClung-Toabe Egg Yolk Agar Composition per liter Pancreatic digest of Yeast Egg yolk emulsion 50 . MgSO4-7H2O 5 solution . pH at 25 C Egg Yolk Emulsion 50 Composition per Chicken egg Whole chicken NaCl solution . Preparation of Egg Yolk Emulsion 50 Soak eggs with 1 100 dilution of saturated mercuric chloride solution for 1 min. Crack eggs