tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 100

Handbook of Microbiological Media, Fourth Edition part 100 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | M16 Agar 985 Preparation of Artificial Seawater Add components to distilled deionized water and bring volume to . Mix thoroughly. Modified Hutner s Basal Salts Composition per liter Nitrilotriacetic NH4 Metals 44 . Preparation of Modified Hutner s Basal Salts Add nitrilotriacetic acid to of distilled deionized water. Dissolve by adjusting pH to with KOH. Add remaining components. Add distilled deionized water to . Metals 44 Composition per Co NO3 Preparation of Metals 44 Add components to distilled deion-ized water and bring volume to . Mix thoroughly. Vitamin Solution Composition per liter D-Calcium Folic acid. Vitamin Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except modified Hutner s basal salts to distilled deionized water and bring volume to . Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature. Aseptically add of sterile modified Hutner s basal salts. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the cultivation and maintenance of Verrucomicrobium spino-sum. M14 Medium Composition per liter Yeast Tris hydroxymethyl Artificial Modified Hutner s basal pH at 25 C Artificial Seawater Composition per liter KBr . NaF . Preparation of Artificial Seawater Add components to distilled deionized water and bring volume to . Mix thoroughly. Modified Hutner s .