tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 46

Handbook of Microbiological Media, Fourth Edition part 46 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Congo Red BHI Agarose Medium 445 Supplement Solution Composition per liter Nicotinamide adenine Vitamin Thiamine Fe NO3 p-Aminobenzoic Source The supplement solution IsoVitaleX enrichment is available from BD Diagnostic Systems. This enrichment may be replaced by supplement VX from BD Diagnostic Systems. Preparation of Supplement Solution Add components to dis-tilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium To of sterile cooled GC agar base aseptically add of sterile cooled hemoglobin solution of sterile supplement solution and of sterile Congo Red solution. Mix thoroughly. Pour into sterile Petri dishes. Use For the isolation and differentiation of virulent and avirulent strains of Shigella Vibrio cholerae Escherichia coli and Neisseria meningitidis. Used for the detection and differentiation of iron-responsive avirulent mutants. Used in the preparation of live vaccines. Used for the differentiation of sensitive Neisseria gonorrhoeae no growth from other Neisseria species growth that are resistant to Congo Red. Congo Red Agar CR Agar Composition per liter Soybean-casein digest Hemoglobin Supplement Congo Red solution . pH at 25 C Soybean-Casein Digest Agar Composition per Pancreatic digest of Papaic digest of soybean Preparation of Soybean-Casein Digest Agar Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pres-sure-121 C. Cool to 45 -50 C. Hemoglobin Solution Composition per Preparation of Hemoglobin Solution Add hemoglobin to .