tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 98

Handbook of Microbiological Media, Fourth Edition part 98 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Low Iron YC Agar 965 Vitamin K Solution Composition per Vitamin Preparation of Vitamin K Solution Add vitamin K1 to of absolute ethanol. Mix thoroughly. Preparation of Medium Add hemin and L-cystine to of NaOH. Mix thoroughly. Add remaining components except agar and gelatin. Bring volume to with distilled deionized water. Mix thoroughly. Gently heat and bring to boiling. In a separate flask add gelatin to of cold distilled deionized water. Gently heat and bring to 70 C. Add gelatin solution to the of basal medium. Mix thoroughly. Add agar. Bring volume to with distilled deionized water. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes. Use For the cultivation of a wide variety of anaerobic bacteria. For the differentiation of anaerobic bacteria based on gelatinase production. After incubation of plates gelatinase activity is determined by the addition of Frazier s reagent. Bacteria that hydrolyze gelatin appear as colonies surrounded by a clear zone. Lombard-Dowell Neomycin Agar Egg Yolk Agar with Neomycin Composition per Neomycin LD Egg yolk MgSO4-7H20 5 solution . pH at 25 C LD Agar Composition per liter Pancreatic digest of Yeast Hemin. NaOH 1V NaOH . Vitamin K1 Preparation of LD Agar Add hemin and L-cystine to of NaOH. Mix thoroughly. Add remaining components. Mix thoroughly. Gently heat and bring to boiling. Vitamin K Solution Composition per Vitamin Preparation of Vitamin K Solution Add vitamin K to of absolute ethanol. Mix thoroughly. Egg Yolk Emulsion Composition Chicken egg Whole chicken Preparation of Egg Yolk Emulsion Soak eggs with 1 100 dilution of saturated mercuric chloride .