tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 97

Handbook of Microbiological Media, Fourth Edition part 97 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Listeria Identification HiVeg Agar Base with PALCAMSelective Supplement 955 Listeria Enrichment HiVeg Broth Modified Composition per liter Plant hydrolysate No. Yeast Plant Nalidixic Acriflavin hydrochloride Trypaflavin . pH at 25 C Source This medium is available as a premixed powder from HiMe-dia. Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the selective enrichment of Listeria species. Listeria Enrichment HiVeg Medium Base with Acriflavine and Nalidixic Acid UVM Composition per liter Plant extract . Plant hydrolysate. Plant peptone No. 3. Yeast extract. Esculin . Nalidixic acid Acriflavine pH at 25 C Source This medium without nalidixic acid or acriflavine solutions is available as a premixed powder from HiMedia. Nalidixic Acid Solution Composition per Nalidixic NaOH solution . Preparation of Nalidixic Acid Solution Add nalidixic acid to of NaOH solution. Mix thoroughly. Filter sterilize. Acriflavine Solution Composition per Preparation of Acriflavine Solution Add acriflavine to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Use freshly prepared solution. Preparation of Medium Add components except nalidixic acid solution and acriflavine solution to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add of sterile nalidixic acid solution. Mix thoroughly. Store at 4 C. Immediately prior to use aseptically add of sterile acriflavine .