tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 95

Handbook of Microbiological Media, Fourth Edition part 95 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | LB Medium with Tetracycline and Ampicillin 935 Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation and maintenance of Escherichia coli. LB Medium with IPTG Medium LB Medium with 50mg of Kanamycin Composition per liter Pancreatic digest of Yeast Kanamycin. Composition per liter Pancreatic digest of Yeast IPTG pH at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of Escherichia coli. pH at 25 C IPTG Solution Composition per IPTG Isopropylthio-P-galactoside . Preparation of IPTG Solution Add IPTG to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except IPTG solution to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Autoclave for 15 min at 15 psi pressure-121 C. Aseptically add sterile IPTG solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the cultivation and maintenance of Escherichia coli. LB Medium with Kanamycin LB Medium with 100mg of Kanamycin ATCC Medium 1468 Composition per liter Pancreatic digest of Yeast Kanamycin. pH at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation and maintenace of Erwinia uredovora. Composition per liter Pancreatic digest of Yeast extract. .