tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 42

Handbook of Microbiological Media, Fourth Edition part 42 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Clostridium grantii Medium 405 Clostridium formicoaceticum Agar Composition per liter Yeast Sodium Fructose NaHCO3 Trace elements solution pH at 25 C Trace Elements Solution SL-4 Composition per liter FeSO4-7H2O. Trace elements solution Trace Elements Solutions SL-6 Composition per liter ZnSO4-7H2O. Na2MoO4-2H2O. Preparation of Trace Elements Solution SL-6 Add components to distilled deionized water and bring volume to . Mix thoroughly. Preparation of Trace Elements Solution SL-4 Add components to distilled deionized water and bring volume to . Mix thoroughly. Fructose Solution Composition per Fructose. Preparation of Fructose Solution Add fructose to distilled deionized water and bring volume to . Mix thoroughly. Sparge under 100 N2 gas for 3 min. Filter sterilize. Store under N2 gas. NaHCO3 Solution Composition per Preparation of NaHCO3 Solution Add NaHCO3 to distilled deionized water and bring volume to . Mix thoroughly. Sparge with 100 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Preparation of Medium Add components except fructose solution and NaHCO3 solution to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Cool to 50 C while sparging with 100 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically and anaerobically add of sterile fructose solution and of sterile NaHCO3 solution. Mix thoroughly. Adjust pH to . Aseptically and anaerobically pour into sterile Petri dishes or distribute into sterile screw-capped bottles under 100 CO2. Use For the cultivation and maintenance of Clostridium formicoaceticum. Clostridium formicoaceticum Broth Composition per liter .