tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 30

Handbook of Microbiological Media, Fourth Edition part 30 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Burk s Medium 285 Burke s Modified Nitrogen-Free Medium with Benzoate Composition per liter Sodium Na2MoO3. pH at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of Pseudomonas species and other microorganisms which can utilize benzoate as sole carbon source. Burkholderia cepacia Agar Composition per liter Sodium Yeast Bile NH4 Phenol Fe NH4 2 SO4 Crystal Selective supplement pH at 25 C Source This medium is available as a premixed powder from Oxoid Unipath. Selective Supplement Solution Composition per Polymyxin 000IU Preparation of Selective Supplement Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except selective supplement solution to distilled deionized water and bring volume to . Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 C. Aseptially add selective supplement solution. Mix thoroughly. Pour into sterile Petri dishes. Use For the selective isolation of Burkholderia cepacia from the respiratory secretions of patients with cystic fibrosis and for routine testing of non-sterile inorganic salt solutions containing preservative. Slow growing B. cepacia can be missed on conventional media such as blood or MacConkey agar due to overgrowth caused by other faster growing organisms found in the respiratory tract of CF patients such as .

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