tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 28

Handbook of Microbiological Media, Fourth Edition part 28 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Bromo Cresol Purple Azide HiVeg Broth 265 Beef Bromcresol Purple pH at 25 C Bromcresol Purple Solution Composition per Bromcresol Ethanol 95 solution . Preparation of Bromcresol Purple Solution Add Bromcresol Purple to of ethanol. Mix thoroughly. Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Distribute into tubes in 12-15mL volumes. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation and differentiation of bacteria based on their ability to ferment glucose. Bacteria that ferment glucose turn the medium yellow. Bromcresol Purple Milk Solids Glucose Agar BCP MS G Agar Composition per Skim milk Bromcresol Purple pH at 25 C Bromcresol Purple Solution Composition per Bromcresol Ethanol 95 solution . Preparation of Bromcresol Purple Solution Add Bromcresol Purple to of ethanol. Mix thoroughly. Preparation of Medium Add skim milk powder and Bromcresol Purple solution to distilled deionized water and bring volume to . Mix thoroughly. Autoclave for 8 min at 11 psi pressure-116 C. Cool to 45 -50 C. In a separate flask add glucose to distilled deionized water and bring volume to . Mix thoroughly. Autoclave for 8 min at 11 psi pressure-116 C. Cool to 45 -50 C. In a third flask add agar to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically combine the three sterile solutions. Mix thoroughly. Aseptically adjust the pH to with sterile 1A HCl. Aseptically distribute into sterile tubes. Allow tubes to cool in a slanted position. Use For the cultivation and differentiation of Trychophyton menta-grophytes Trychophyton rubrum and Microsporum persicolor. Bromcresol Purple Milk Yeast Extract with .