tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 25

Handbook of Microbiological Media, Fourth Edition part 25 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Bolton Broth 235 ACES buffer 2- 2-amino-2-oxoethyl - amino -ethane sulfonic acid . Charcoal Fe4 P2O7 Antibiotic L-Cysteine-HCl-H2O pH at 25 C Antibiotic Inhibitor Composition per Polymyxin 000U Preparation of Antibiotic Inhibitor Add components to dis-tilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. L-Cysteine-HCl-H2O Solution Composition per Preparation of L-CysteinefHCl H2O Solution Add l-cysteine-HCl-H2O to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except antibiotic inhibitor and L-cysteine-HCl-H2O solution to distilled deionized water and bring volume to . Mix thoroughly. Adjust medium to pH with 1A KOH. Heat gently and bring to boiling for 1 min. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 -55 C. Add of the sterile L-cysteine-HCl-H2O solution and of the sterile antibiotic solution. Mix thoroughly. Pour into sterile Petri dishes with constant agitation to keep charcoal in suspension. Use For the selective isolation and cultivation ofLegionella pneumophila and other Legionella species. BMS Agar Composition per liter L-Malic Potato extract Bromthymol Vitamin pH at 25 C Potato Extract Solution Composition per liter Potatoes washed peeled and Preparation of Potato Extract Solution Wash peel and slice several large potatoes. Place the poltato slices in a gauze bag. Place the bag with the potatoes in of distilled deionized water. Boil for 30 min. Filter through cotton. Bromthymol Blue Solution Composition per Bromthymol Ethanol 95 . Preparation of Bromthymol Blue Solution Add Bromthymol Blue to of .