tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 23

Handbook of Microbiological Media, Fourth Edition part 23 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | BHI 3 Medium 215 Peptic digest of animal pH at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of Corynebacterium spp. Streptomyces flocculus Mycobacterium spp. Nocardia spp. Rhodococcus spp. Dermato-philus congolensis and Gordonia amicalis. BHI with Glycerol and Reducing Agents DSMZ Medium 215c Composition per liter Pancreatic digest of Brain heart solids from Peptic digest of animal Glycerol L-Cysteine-HCl-Na2S pH at 25 C Glycerol Solution Composition per Preparation of Glycerol Solution Add glycerol to distilled deionized water and bring volume to . Mix thoroughly. L-Cysteine-HCl-Na2S Solution Composition per Preparation of L-Cysteine-HCl-Na2S Solution Add l-cysteine-HCl to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to 11 with NaOH. Add Na2S-9H2O. Mix thoroughly. Bring volume to with distilled deionized water. Gently heat and bring to boiling under 100 N2. Cool to 25 C under 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Preparation of Medium Add components except L-cysteine-HCl-Na2S solution to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling under 100 N2. Cool to 25 C under 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically and anaerobically under 100 N2 add of l-cysteine-HCl-Na2S solution. Mix thoroughly. Aseptically under 100 N2 distribute to tubes. Alternately distribute amounts of the medium without L-cysteine-HCl-Na2S solution to tubes prior to autoclaving. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically and .

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