tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 74

Handbook of Microbiological Media, Fourth Edition part 74 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | GC Agar with Streptomycin and Chloramphenicol 725 phenicol solution and of sterile tetracycline solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the cultivation and maintenance of Haemophilus parainflu-enzae. GC Agar with Defined Supplements GC agar Defined supplements pH at 25 C GC Agar Base Composition per liter Pancreatic digest of Peptic digest of animal Source GC agar base is available as a premixed powder from BD Diagnostic Systems. This base may be replaced by GC medium base available from BD Diagnostic Systems. Preparation of GC Agar Base Add components to distilled de-ionized water and bring volume to . Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Defined Supplements Solution Composition per Glutamine. Fe NO3 Preparation of Defined Supplements Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium To of sterile GC agar base aseptically add of sterile defined supplements solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the cultivation and maintenance of Neisseria gonorrhoeae. GC Agar with Penicillin G Composition per GC medium base Hemoglobin Supplement Penicillin G pH at 25 C GC Medium Base 2X Composition per Proteose peptone No. Source GC medium base is available as a premixed powder from BD Diagnostic Systems. Preparation of GC Medium Base 2X Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat until boiling. .