tailieunhanh - Báo cáo khoa học: Vanadium-induced apoptosis of HaCaT cells is mediated by c-fos and involves nuclear accumulation of clusterin

Vanadium exerts a variety of biological effects, including antiproliferative responses through activation of the respective signaling pathways and the generation of reactive oxygen species. As epidermal cells are exposed to environmental insults, human keratinocytes (HaCaT) were used to investi-gate the mechanism of the antiproliferative effects of vanadyl(IV) sulfate (VOSO4). | Vanadium-induced apoptosis of HaCaT cells is mediated by c-fos and involves nuclear accumulation of clusterin Soultana Markopoulou1 Evangelos Kontargiris1 Christina Batsi1 Theodore Tzavaras2 loannis Trougakos3 4 David A. Boothman5 Efstathios S. Gonos3 and Evangelos Kolettas1 6 1 Cellular and Molecular Physiology Unit Laboratory of Physiology Schoolof Medicine University of loannina Greece 2 Laboratory of GeneralBiology Schoolof Medicine University of Ioannina Greece 3 Laboratory of Cellular and Molecular Ageing Institute of BiologicalResearch and Biotechnology NationalHellenic Research Foundation Athens Greece 4 Department of CellBiology and Biophysics Faculty of Biology University of Athens Greece 5 Laboratory of Molecular Stress Responses Program in CellStress and Cancer Nanomedicine Department of Oncology University of Texas Southwestern MedicalCentre at Dallas TX USA 6 BiomedicalResearch Institute Foundation of Research and Technology BRI-FORTH Ioannina Greece Keywords apoptosis Bcl-2 c-fos clusterin CLU vanadyl IV sulfate Correspondence E. Kolettas Cellular and Molecular Physiology Unit Laboratory of Physiology Schoolof Medicine University of Ioannina 45110 Ioannina Greece Fax 30 26510 97850 Tel 30 26510 97578 E-mail ekoletas@ These authors contributed equally to this work Received 22 January 2009 revised 28 April 2009 accepted 13 May 2009 doi Vanadium exerts a variety of biological effects including antiproliferative responses through activation of the respective signaling pathways and the generation of reactive oxygen species. As epidermal cells are exposed to environmental insults human keratinocytes HaCaT were used to investigate the mechanism of the antiproliferative effects of vanadyl IV sulfate VOSO4 . Treatment of HaCaT cells with VOSO4 inhibited proliferation and induced apoptosis in a dose-dependent manner. Inhibition of proliferation was associated with downregulation of cyclins D1 and E E2F1 and the .