tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 12

Handbook of Microbiological Media, Fourth Edition part 12 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Anaerobic HiVeg Agar Base with Egg Yolk Emulsion 105 Preparation of Medium Add components except egg yolk emulsion to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add sterile egg yolk emulsion. Mix thoroughly. Pour into sterile Petri dishes. Use For the cultivation of Clostridium perfringens from foods. Anaerobic D-Gluconate Medium Composition per liter Pancreatic digest of Yeast NH4 NaHCO3 pH at 25 C NaHCO3 Solution Composition per Preparation of NaHCO3 Solution Add NaHCO3 to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except NaHCO3 solution to distilled deionized water and bring volume to . Prepare anaerobically under 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically add of the sterile NaHCO3 solution. Mix thoroughly. Adjust pH to . Use For the cultivation and maintenance of microorganisms that can utilize D-gluconate as a carbon source such as Bacteroides pectinophi-lus. Anaerobic Glucuronic Acid Medium Composition per liter Pancreatic digest of Yeast Glucuronic NH4 2SO4. Resazurin . NaHCO3 pH at 25 C NaHCO3 Solution Composition per Preparation of NaHCO3 Solution Add NaHCO3 to distilled de-ionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except NaHCO3 solution to distilled deionized water and bring volume to . Pre pare anaerobically under 100 N2. Autoclave for 15 min at 15 psi