tailieunhanh - Báo cáo khoa học: Glutathione transferases in bacteria

Bacterial glutathione transferases (GSTs) are part of a superfamily of enzymes that play a key role in cellular detoxification. GSTs are widely dis-tributed in prokaryotes and are grouped into several classes. Bacterial GSTs are implicated in a variety of distinct processes such as the biodegra-dation of xenobiotics, protection against chemical and oxidative stresses and antimicrobial drug resistance. | REVIEW ARTICLE Glutathione transferases in bacteria Nerino Allocati1 Luca Federici1 2 Michele Masulli1 and Carmine Di Ilio1 2 1 Dipartimento di Scienze Biomediche Universita G. d Annunzio Chieti Italy 2 . Centro Studi per l Invecchiamento Universita G. d Annunzio Chieti Italy Keywords bacterial glutathione transferase chemical stress DCM dehalogenase detoxification drug resistance fosfomycin resistance proteins glutathione HCCA isomerase oxidative stress TCHQ dehalogenase Correspondence N. Allocati Dipartimento di Scienze Biomediche Universita G. d Annunzio Via dei Vestini 31 I-66013 Chieti Italy Fax 39 0871 355 5282 Tel 39 0871 355 5281 E-mail allocati@ Bacterial glutathione transferases GSTs are part of a superfamily of enzymes that play a key role in cellular detoxification. GSTs are widely distributed in prokaryotes and are grouped into several classes. Bacterial GSTs are implicated in a variety of distinct processes such as the biodegradation of xenobiotics protection against chemical and oxidative stresses and antimicrobial drug resistance. In addition to their role in detoxification bacterial GSTs are also involved in a variety of distinct metabolic processes such as the biotransformation of dichloromethane the degradation of lignin and atrazine and the reductive dechlorination of pentachlorophenol. This review article summarizes the current status of knowledge regarding the functional and structural properties of bacterial GSTs. Received 25 July 2008 revised 8 October 2008 accepted 14 October 2008 doi Introduction Glutathione transferases GSTs EC constitute a protein superfamily that is involved in cellular detoxification against harmful xenobiotics and endo-biotics 1-3 . One common feature of all GSTs is their ability to catalyse nucleophilic attack by the tripeptide glutathione GSH on the electrophilic groups of a wide range of hydrophobic toxic compounds thus promoting their excretion from the cell