tailieunhanh - Laboratory Exercises in Microbiology - part 4

an toàn Hãy cẩn thận với ngọn lửa ghi Bunsen. Không có pipetting miệng. Các thuốc thử oxidase là ăn da. Tránh tiếp xúc với mắt và da. Trong trường hợp tiếp xúc, ngay lập tức rửa mắt hoặc da với nhiều nước trong vòng ít nhất 15 phút. | Harley-Prescott Laboratory Exercises in Microbiology Fifth Edition IV. Biochemical Activities of Bacteria The McGraw-Hill Companies 2002 20. Carbohydrates I Fermentation andBGalactosidase Activity Review Questions 1. Define fermentation. 2. Do all microorganisms produce the same end product from pyruvate Explain your answer. 3. What is the purpose of the phenol red or bromcresol purple in the fermentation tube 4. What is the function of the Durham tube in the fermentation tube 5. What are some of the metabolic end products produced by the different microorganisms used in this experiment 6. What is the color of phenol red at an acid pH 7. What is the function of P-galactosidase 132 Biochemical Activities of Bacteria Harley-Prescott Laboratory Exercises in Microbiology Fifth Edition IV. Biochemical Activities of Bacteria 21. Carbohydrates II Triple Sugar Iron Agar Test The McGraw-Hill Companies 2002 EXERCISE Carbohydrates II Triple Sugar Iron Agar Test SAFETY CONSIDERATIONS Be careful with the Bunsen burner flame. Be careful when working with these bacteria especially Shigella dysenteriae as they are known pathogens. Keep all culture tubes upright in a test-tube rack or in empty cans. Materials per Student 24- to 48-hour tryptic soy broth cultures of Alcaligenes faecalis ATCC 8750 Escherichia coli ATCC 11229 Proteus vulgaris ATCC 13315 Pseudomonas aeruginosa ATCC 10145 and Shigella flexneri ATCC 12661 5 triple sugar iron agar slants Bunsen burner inoculating needle incubator set at 35 C test-tube rack Learning Objectives Each student should be able to 1. Understand the biochemical reactions involved in the triple sugar iron agar test 2. Differentiate among members of the family Enterobacteriaceae 3. Distinguish between the Enterobacteriaceae and other intestinal bacteria 4. Perform a TSI test Suggested Reading in Textbook 1. Carbohydrate catabolism section . 2. The Enterobacteriaceae section . Pronunciation Guide Alcaligenes faecalis al-kah-LIJ-e-neez .