tailieunhanh - Laboratory Exercises in Microbiology - part 10

Sau khi ủ, thực hiện các slide với kính hiển vi, bằng cách sử dụng các mục tiêu năng lượng thấp. Thêm một vài giọt màu xanh methylene methanol vết các cấu trúc khác nhau. (Rượu cần thiết để làm mềm các thành tế bào và cho phép các vết bẩn để vào.) | Harley-Prescott Appendixes Laboratory Exercises in Microbiology Fifth Edition A. Dilutions with Sample Problems The McGraw-Hill Companies 2002 Problem 6 C1 70 c2 50 70 _ 45 v _ 50 X 45 2 - - - X z-X ml V2 45 ml 50 X 70 V1 X Thus ml of 70 alcohol ml of H2O 45 ml of a 50 alcohol solution. Problem 7 C1 20 c2 1 20 _ 250 _ 2 _ _ - - 20X 250 X ml V2 150 ml 1 X V1 X Thus ml of a 20 solution ml of broth 250 ml of a 1 dextrose broth solution. Problem 8 C1 5 c2 2 V2 45 ml V1 X - 45 ml V 90- X 1Rm1 - Y X _ X _ 18 ml 2 X 5 Thus 18 ml of 5 27 ml of saline 45 ml of a 2 red blood cell suspension. Problem 9 C1 C2 - - X- X 125 ml V2 X 25 ml V1 25 ml Thus prepare a total volume of 125 of a solution from 25 ml of solution by adding 100 ml of diluent to the latter. Problem 10 C1 95 C2 X I-- 3 ml 38X 950 X 25 V2 38 ml X 10 ml V1 10 ml Problem 11 C1 10 C2 X ---È T51 ml- 15X 30 X 2 V2 15 ml X 3 ml V1 3 ml 3 12 15 ml total volume Problem 12 In order to make all units equal you have to convert 10 grams to milligrams. stock solution 10 000 mg ml final concentration 2 mg ml IC _ 10 000 mg ml ----------- -- 5 000 FC 2 mg ml Thus the dilution factor is 5 000. To perform a 1 5000 dilution Step 1 1 ml of 10 000 mg ml stock solution protein 49 ml of diluent 1 50 dilution 200 mg ml Step 2 1 ml of 200 mg ml 9 ml of diluent 1 10 dilution 20 mg ml Step 3 1 ml of 20 mg ml 9 ml of diluent 1 10 dilution 2 mg ml To check to make sure the correct dilution was made 50 X 10 X 10 5 000 424 Appendix A Dilutions with Sample Problems Harley-Prescott Appendixes Laboratory Exercises in Microbiology Fifth Edition A. Dilutions with Sample Problems The McGraw-Hill Companies 2002 Problem 13 Make all units the same. 10 mg X 1 000 Fg mg 10 000 Fg stock solution 10 000 Fg ml final concentration Fg ml _C DF 10 000 pg 20000 FC Fg ml Thus the dilution factor is 20 000. Step 1 1 ml of 10 000 Fg ml 19 ml of diluent 1 20 dilution 500 Fg ml 10 000 20