tailieunhanh - Báo cáo y học: "Integrated miRNA and mRNA expression profiling of mouse mammary tumor models identifies miRNA signatures associated with mammary tumor lineage"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Integrated miRNA and mRNA expression profiling of mouse mammary tumor models identifies miRNA signatures associated with mammary tumor lineage. | Genome Biology Integrated miRNA and mRNA expression profiling of mouse mammary tumor models identifies miRNA signatures associated with mammary tumor lineage Zhu et al. Zhu et al. Genome Biology 2011 12 R77 http 2011 12 8 R77 16 August 2011 2 Bk Med Central Zhu et al. Genome Biology 2011 12 R77 http 2011 12 8 R77 Genome Biology RESEARCH Open Access Integrated miRNA and mRNA expression profiling of mouse mammary tumor models identifies miRNA signatures associated with mammary tumor lineage Min Zhu1 Ming Yi2 Chang Hee Kim3 Chuxia Deng4 Yi Li5 Daniel Medina6 Robert M Stephens2 and Jeffrey E Green1 Abstract Background MicroRNAs miRNAs are small non-coding endogenous RNAs involved in regulating gene expression and protein translation. miRNA expression profiling of human breast cancers has identified miRNAs related to the clinical diversity of the disease and potentially provides novel diagnostic and prognostic tools for breast cancer therapy. In order to further understand the associations between oncogenic drivers and miRNA expression in sub-types of breast cancer we performed miRNA expression profiling on mammary tumors from eight well-characterized genetically engineered mouse GEM models of human breast cancer including MMTV-H-Ras -Her2 neu -c-Myc -PymT -Wnt1 and C3 1 SV40 T t-antigen transgenic mice BRCA1fl fl p53 - MMTV-cre knockout mice and the p53fl fl MMTV-cre transplant model. Results miRNA expression patterns classified mouse mammary tumors according to luminal or basal tumor subtypes. Many miRNAs found in luminal tumors are expressed during normal mammary development. miR-135b miR-505 and miR-155 are expressed in both basal human and mouse mammary tumors and many basal-associated miRNAs have not been previously characterized. miRNAs associated with the initiating oncogenic event driving tumorigenesis were also identified. miR-10b -148a -150 -199a and -486 were only expressed in normal mammary epithelium and not tumors .