tailieunhanh - Báo cáo lâm nghiệp: "In vitro clonal propagation of Acacia mangium Willd. and its evaluation of genetic stability through RAPD marker"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí lâm nghiệp đề tài: In vitro clonal propagation of Acacia mangium Willd. and its evaluation of genetic stability through RAPD marker. | Ann. For. Sci. 61 2004 381-386 381 INRA EDP Sciences 2004 DOI forest 2004031 Note In vitro clonal propagation of Acacia mangium Willd. and its evaluation of genetic stability through RAPD marker Rashmi M. Nanda Premananda Das Gyana Ranjan Rout Plant Biotechnology Division Plant Tissue Culture Laboratory Regional Plant Resource Centre Bhubaneswar- 751 015 Orissa India Received 28 October 2002 accepted 5 June 2003 Abstract - An in vitro propagation of a tropical leguminous tree Acacia mangium has been established. Induction of bud sprout was obtained from mature nodal explants of 10-years-old tree of Acacia mangium on Murashige and Skoog MS 10 basal medium supplemented with 6-benzylaminopurine BAP mg L gibberellic acid GA3 mg L and indole-3-acetic acid IAA mg L. The rate of multiplication was obtained on MS medium supplemented with mg L BAP mg L IAA and 100 mg L adenine sulfate Ads . The multiplication rate varied from 1 to 8 depending on the growth regulators used. Excised shoots were rooted on half-strength MS basal salts supplemented with mg L indole-3-butyric acid IBA or indole-3-acetic acid IAA and 20 g L w v sucrose after 13-14 days of culture. The micropropagated plantlets have been acclimatized and successfully transferred to soil. The micropropagated plantlets appeared morphologically similar to the mother plant. No variation was detected among the micropropagated plants by the use of Randomly Amplified Polymorphic DNA RAPD markers. in vitro legume tree multiplication micro-propagation RAPD marker Résumé - Propagation de clones in vitro d Acacia mangium Willd. et evaluation de leur stabilité génétique avec un marquage RADP. On a realise la propagation in vitro d un arbre Acacia mangium de la famille des légumineuses. L induction de bourgeons débourrés a été obtenue à partir d explants de bois aoũté prélevés sur des arbres âgés de 10 ans cultivés dans le milieu de base de Murashige et Skoog MS auquel on a ajouté de la .