tailieunhanh - Báo cáo y học: "Heterochronic evolution reveals modular timing changes in budding yeast transcriptomes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Heterochronic evolution reveals modular timing changes in budding yeast transcriptomes. | Simola et al. Genome Biology 2010 11 R105 http 2010 11 10 R105 Genome Biology RESEARCH Open Access Heterochronic evolution reveals modular timing changes in budding yeast transcriptomes 11 1 Daniel F Simola Chantal Francis Paul D Sniegowski Junhyong Kim Abstract Background Gene expression is a dynamic trait and the evolution of gene regulation can dramatically alter the timing of gene expression without greatly affecting mean expression levels. Moreover modules of co-regulated genes may exhibit coordinated shifts in expression timing patterns during evolutionary divergence. Here we examined transcriptome evolution in the dynamical context of the budding yeast cell-division cycle to investigate the extent of divergence in expression timing and the regulatory architecture underlying timing evolution. Results Using a custom microarray platform we obtained 378 measurements for 6 263 genes over 18 timepoints of the cell-division cycle in nine strains of S. cerevisiae and one strain of S. paradoxus. Most genes show significant divergence in expression dynamics at all scales of transcriptome organization suggesting broad potential for timing changes. A model test comparing expression level evolution versus timing evolution revealed a better fit with timing evolution for 82 of genes. Analysis of shared patterns of timing evolution suggests the existence of seven dynamically-autonomous modules each of which shows coherent evolutionary timing changes. Analysis of transcription factors associated with these gene modules suggests a modular pleiotropic source of divergence in expression timing. Conclusions We propose that transcriptome evolution may generally entail changes in timing heterochrony rather than changes in levels heterometry of expression. Evolution of gene expression dynamics may involve modular changes in timing control mediated by module-specific transcription factors. We hypothesize that genome-wide gene regulation may utilize a general .