tailieunhanh - Báo cáo y học: "An atlas of bovine gene expression reveals novel distinctive tissue characteristics and evidence for improving genome annotation"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: An atlas of bovine gene expression reveals novel distinctive tissue characteristics and evidence for improving genome annotation. | Harhay et al. Genome Biology 2010 11 R102 http 2010 11 10 R102 w Genome Biology RESEARCH Open Access An atlas of bovine gene expression reveals novel distinctive tissue characteristics and evidence for improving genome annotation 1 1 2 31 Gregory P Harhay Timothy PL Smith Leeson J Alexander Christian D Haudenschild John W Keele I l r m I I I I I m I I ỉ4 5 c rG c r rr r- I r5 I I I ỉ D I f I I 5 h f D rG ỉ m 6 c I ir I D rl r6 Lakshmi K Matukumalli Steven G Schroeder Curtis P Van lassell Cathy R Gresham Susan M Bridges Shane C Burgess7 Tad S Sonstegard5 Abstract Background A comprehensive transcriptome survey or gene atlas provides information essential for a complete understanding of the genomic biology of an organism. We present an atlas of RNA abundance for 92 adult juvenile and fetal cattle tissues and three cattle cell lines. Results The Bovine Gene Atlas was generated from million unique digital gene expression tag sequences million total raw tag sequences from which million unique tag sequences were identified that mapped to the draft bovine genome accounting for 85 of the total raw tag abundance. Filtering these tags yielded 87 764 unique tag sequences that unambiguously mapped to 16 517 annotated protein-coding loci in the draft genome accounting for 45 of the total raw tag abundance. Clustering of tissues based on tag abundance profiles generally confirmed ontology classification based on anatomy. There were 5 429 constitutively expressed loci and 3 445 constitutively expressed unique tag sequences mapping outside annotated gene boundaries that represent a resource for enhancing current gene models. Physical measures such as inferred transcript length or antisense tag abundance identified tissues with atypical transcriptional tag profiles. We report for the first time the tissue-specific variation in the proportion of mitochondrial transcriptional tag abundance. Conclusions The Bovine Gene Atlas is the deepest and broadest