tailieunhanh - Báo cáo y học: "Genome-wide analysis of mRNA decay patterns during early Drosophila development"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Genome-wide analysis of mRNA decay patterns during early Drosophila development. | Thomsen et al. Genome Biology 2010 11 R93 http 2010 11 9 R93 Genome Biology RESEARCH Open Access Genome-wide analysis of mRNA decay patterns during early Drosophila development G 4-z f s I I . rvn r Z 1 2 G I z A z l Z z r 3 4 G z h f I z l V I s Z t s 5 6 A r I Cz i Z t I_11 11 z3 4 f I I I z l I z D A I z 1 z 1 2 Stefan Thomsen Simon Anders Sarath Chandra Janga Wolfgang Huber Claudio R Alonso Abstract Background The modulation of mRNA levels across tissues and time is key for the establishment and operation of the developmental programs that transform the fertilized egg into a fully formed embryo. Although the developmental mechanisms leading to differential mRNA synthesis are heavily investigated comparatively little attention is given to the processes of mRNA degradation and how these relate to the molecular programs controlling development. Results Here we combine timed collection of Drosophila embryos and unfertilized eggs with genome-wide microarray technology to determine the degradation patterns of all mRNAs present during early fruit fly development. Our work studies the kinetics of mRNA decay the contributions of maternally and zygotically encoded factors to mRNA degradation and the ways in which mRNA decay profiles relate to gene function mRNA localization patterns translation rates and protein turnover. We also detect cis-regulatory sequences enriched in transcripts with common degradation patterns and propose several proteins and microRNAs as developmental regulators of mRNA decay during early fruit fly development. Finally we experimentally validate the effects of a subset of cis-regulatory sequences and frans-regulators in vivo. Conclusions Our work advances the current understanding of the processes controlling mRNA degradation during early Drosophila development taking us one step closer to the understanding of mRNA decay processes in all animals. Our data also provide a valuable resource for further experimental and .