tailieunhanh - Báo cáo y học: " The head-regeneration transcriptome of the planarian Schmidtea mediterranea"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: The head-regeneration transcriptome of the planarian Schmidtea mediterranea. | Sandmann et al. Genome Biology 2011 12 R76 http 2011 12 8 R76 Genome Biology RESEARCH Open Access The head-regeneration transeriptome of the planarian Schmidtea mediterranea Thomas Sandmann1 2 Matthias C Vogg 3 Suthira Owlarn3 Michael Boutros1 4 and Kerstin Bartscherer3 Abstract Background Planarian flatworms can regenerate their head including a functional brain within less than a week. Despite the enormous potential of these animals for medical research and regenerative medicine the mechanisms of regeneration and the molecules involved remain largely unknown. Results To identify genes that are differentially expressed during early stages of planarian head regeneration we generated a de novo transcriptome assembly from more than 300 million paired-end reads from planarian fragments regenerating the head at 16 different time points. The assembly yielded 26 018 putative transcripts including very long transcripts spanning multiple genomic supercontigs and thousands of isoforms. Using shortread data from two platforms we analyzed dynamic gene regulation during the first three days of head regeneration. We identified at least five different temporal synexpression classes including genes specifically induced within a few hours after injury. Furthermore we characterized the role of a conserved Runx transcription factor smed-runt-likel. RNA interference RNAi knockdown and immunofluorescence analysis of the regenerating visual system indicated that smed-runt-likel encodes a transcriptional regulator of eye morphology and photoreceptor patterning. Conclusions Transcriptome sequencing of short reads allowed for the simultaneous de novo assembly and differential expression analysis of transcripts demonstrating highly dynamic regulation during head regeneration in planarians. Background The limited regenerative capabilities of humans call for therapies that can replace or heal wounded tissues. The tre atment of neurodegenerative diseases has been a major .