tailieunhanh - Báo cáo y học: "Dynamic reprogramming of chromatin accessibility during Drosophila embryo development"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Dynamic reprogramming of chromatin accessibility during Drosophila embryo development. | Thomas et al. Genome Biology 2011 12 R43 http 2011 12 5 R43 Genome Biology RESEARCH Open Access Dynamic reprogramming of chromatin accessibility during Drosophila embryo development Qosỉn c1 t Yi n-V inn I i2t Dotor I c hn1 Q nHcirr m1 Dr iHcirl E THi irmuti1 TFiCircico I r 1 jedn inumds AidUiuny LI retei J Jduu nici Idid Sdndstiom nobeit E II luimdn ineiesd I Cdifield 12 2 2 2 Eiikd Giste Willidm Fishei Ann Hdmmonds Susdn E Celnikei Mdik D Biggin dnd John A Stdmdtoydnnopoulos1 Abstract Background The development of complex organisms is believed to involve progressive restrictions in celluldi fdte. Understanding the scope dnd fedtuies of chramdtin dyndmics during embiyogenesis dnd identifying ieguldtoiy elements impoitdnt foi directing developmentdl processes iemdin key godls of developmentdl biology. Results We used in vivo DNdsel sensitivity to mdp the locdtions of ieguldtoiy elements dnd explore the chdnging chiomdtin ldndscdpe duiing the fiist 11 houis of Drosophila embiyonic development. We identified thousdnds of conseived developmentdlly dyndmic distdl DNdsel hypeisensitive sites dssocidted with spdtidl dnd tempoidl expression pdtteining of linked genes dnd with ldige regions of chiomdtin pldsticity. We obseived d nedily unifoim bdldnce between developmentdlly up- dnd down-ieguldted DNdsel hypeisensitive sites. Andlysis of piomotei chiomdtin dichitectuie ievedled d novel iole foi cldssicdl coie piomotei sequence elements in directing temporally ieguldted chiomdtin remodeling. Anothei unexpected fedtuie of the chiomdtin ldndscdpe wds the presence of locdlized dccessibility ovei mdny piotein-coding regions subsets of which weie developmentdlly ieguldted oi dssocidted with the tidnsciiption of genes with piominent mdteindl nNA contiibutions in the bldstodeim. Conclusions Oui results provide d globdl view of the iich dnd dyndmic chiomdtin ldndscdpe of edily dnimdl development ds well ds novel insights into the oigdnizdtion of developmentdlly .