tailieunhanh - Báo cáo y học: "ISsaga is an ensemble of web-based methods for high throughput identification and semiautomatic annotation of insertion sequences in prokaryotic genomes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: ISsaga is an ensemble of web-based methods for high throughput identification and semiautomatic annotation of insertion sequences in prokaryotic genomes. | Varani et al. Genome Biology 2011 12 R30 http 2011 12 3 R30 Genome Biology METHOD Open Access ISsaga is an ensemble of web-based methods for high throughput identification and semiautomatic annotation of insertion sequences in prokaryotic genomes Alessandro M Varani Patricia Siguier Edith Gourbeyre Vincent Charneau and Mick Chandler Abstract Insertion sequences ISs play a key role in prokaryotic genome evolution but are seldom well annotated. We describe a web application pipeline ISsaga http ISsaga that provides computational tools and methods for high-quality IS annotation. It uses established ISfinder annotation standards and permits rapid processing of single or multiple prokaryote genomes. ISsaga provides general prediction and annotation tools information on genome context of individual ISs and a graphical overview of IS distribution around the genome of interest. Background The growing number of completely sequenced bacterial and archaeal genomes are making important contributions to understanding genome structure and evolution. Annotation of gene content and genome comparison have also provided much valuable information and key insights into how prokaryotes are genetically tailored to their lifestyles. The rate at which sequenced prokaryotic genomes and metagenomes are accumulating is constantly increasing with the development of new high-throughput sequencing techniques. The resulting mass of data should provide an unparalleled opportunity to achieve a better understanding of prokaryotes. High quality genome annotation together with a standardized nomenclature is an essential requirement for this since most proteins identified from these sequencing projects will probably never be characterized biochemically 1 . Unfortunately expert genome annotation is fast becoming a bottleneck in genomics 2 . A crucial example of an annotation bottleneck concerns insertion sequences ISs the smallest and simplest .