tailieunhanh - Báo cáo khoa học: Bioimaging of the unbalanced expression of microRNA9 and microRNA9* during the neuronal differentiation of P19 cells

The effects of tyramine, serotonin and benzalkonium on the esterase and aryl acylamidase activities of wild-type human butyrylcholinesterase and its peripheral anionic site mutant, D70G, were investigated. The kinetic study was carried out under steady-state conditions with neutral and positively charged aryl acylamides. | ỊFEBS Journal Bioimaging of the unbalanced expression of microRNA9 and microRNA9 during the neuronal differentiation of P19 cells Mee Hyang Ko1 2 3 Soonhag Kim2 4 Do Won Hwang1 2 3 Hae Young Ko2 3 Young Ha Kim2 3 and Dong Soo Lee2 4 1 Programs in Neuroscience SeoulNationalUniversity Korea 2 Department of Nuclear Medicine SeoulNationalUniversity College of Medicine Korea 3 Laboratory of Molecular Imaging and Therapy of Cancer Research Institute SeoulNationalUniversity College of Medicine Korea 4 MedicalResearch Center SeoulNationalUniversity College of Medicine Korea Keywords bioimaging Luciferase microRNA microRNA9 and microRNA9 neurogenesis Correspondence S. Kim Department of Nuclear Medicine MedicalResearch Center SeoulNational University College of Medicine 28 Yongon-dong Jongno-gu Seoul110 744 Korea Fax 82 2 3668 7090 Tel 82 2 3668 7028 E-mail kimsoonhag@ D. S. Lee Department of Nuclear Medicine MedicalResearch Center SeoulNational University College of Medicine 28 Yongon-dong Jongno-gu Seoul 110 744 Korea Fax 82 2 3668 7090 Tel 82 2 2072 2501 E-mail dsl@ These authors contributed equally to this work Received 18 January 2008 revised 10 March 2008 accepted 17 March 2008 doi Generally the 3 -end of the duplex microRNA miR precursor pre-miR is known to be stable in vivo and serve as a mature form of miR. However both the 3 -end miR9 and 5 -end miR9 of a brain-specific miR9 have been shown to function biologically in brain development. In this study real-time PCR analysis and in vitro in vivo bioluminescent imaging demonstrated that the upstream region of a primary miR9-1 pri-miR9-1 can be used to monitor the highly expressed pattern of endogenous pri-miR9-1 during neurogenesis and that the Luciferase reporter gene can image the unequal expression patterns of miR9 and miR9 seen during the neuronal differentiation of P19 cells. This demonstrates that our bioimaging system can be used to study the .

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