tailieunhanh - Báo cáo khoa học: Molecular determinants of ligand specificity in family 11 carbohydrate binding modules – an NMR, X-ray crystallography and computational chemistry approach

The direct conversion of plant cell wall polysaccharides into soluble sugars is one of the most important reactions on earth, and is performed by cer-tain microorganisms such as Clostridium thermocellum(Ct). These organ-isms produce extracellular multi-subunit complexes (. cellulosomes) comprising a consortium of enzymes, which contain noncatalytic carbohy-drate-binding modules (CBM) that increase the activity of the catalytic module. | ễFEBS Journal Molecular determinants of ligand specificity in family 11 carbohydrate binding modules - an NMR X-ray crystallography and computational chemistry approach Aldino Viegas1 Natercia F. Bras2 Nuno M. F. S. A. Cerqueira2 Pedro Alexandrino Fernandes2 Jose A. M. Prates3 Carlos M. G. A. Fontes3 Marta Bruix4 Maria Joao Romao1 Ana Luisa Carvalho1 Maria Joao Ramos2 Anjos L. Macedo1 and Eurico J. Cabrita1 1 REQUIMTE-CQFB Departamento de Quimica Faculdade de Ciencias e Tecnologia Universidade Nova de Lisboa Caparica Portugal 2 REQUIMTE Departamento de Quimica Faculdade de Ciencias do Porto Portugal 3 Centro Interdisciplinar de Investigacao em Sanidade Animal Faculdade de Medicina Veterinaria Lisbon Portugal 4 Instituto de Quimica Fisica Rocasolano CSIC Madrid Spain Keywords cellulosome Clostridium thermocellum CÍCBM11 STD-NMR molecular modelling X-ray crystallography Correspondence E. J. Cabrita REQUIMTE-CQFB Departamento de Quimica Faculdade de Ciencias e Tecnologia Universidade Nova de Lisboa 2829-516 Caparica Portugal Fax 351 212948550 Tel 351 212948358 E-mail ejc@ M. J. Ramos REQUIMTE Departamento de Quimica Faculdade de Ciencias do Porto 4169-007 Porto Portugal Fax 351 226082959 Tel 351 226082806 E-mail mjramos@ A. L. Carvalho REQuImTE-CQFB Departamento de Quimica Faculdade de Ciencias e Tecnologia Universidade Nova de Lisboa 2829-516 Caparica Portugal Fax 351 212948550 Tel 351 212948300 E-mail alcarvalho@ These authors contributed equally to this work The direct conversion of plant cell wall polysaccharides into soluble sugars is one of the most important reactions on earth and is performed by certain microorganisms such as Clostridium thermocellum Ct . These organisms produce extracellular multi-subunit complexes . cellulosomes comprising a consortium of enzymes which contain noncatalytic carbohydrate-binding modules CBM that increase the activity of the catalytic module. In the present study we describe a combined .