tailieunhanh - Báo cáo khoa học: Analysis of the sugar-binding specificity of mannose-binding-type Jacalin-related lectins by frontal affinity chromatography – an approach to functional classification

The Jacalin-related lectin (JRL) family comprises galactose-binding-type (gJRLs) and mannose-binding-type (mJRLs) lectins. Although the docu-mented occurrence of gJRLs is confined to the family Moraceae, mJRLs are widespread in the plant kingdom. | ỊFEBS Journal Analysis of the sugar-binding specificity of mannose-binding-type Jacalin-related lectins by frontal affinity chromatography - an approach to functional classification Sachiko Nakamura-Tsuruta1 Noboru Uchiyama1 Willy J. Peumans2 Els J. M. Van Damme2 Kiichiro Totani3 Yukishige Ito3 and Jun Hirabayashi1 1 Lectin Application and Analysis Team Research Center for MedicalGlycoscience National institute of Advanced Industrial science and Technology Tsukuba Japan 2 Department of Molecular Biotechnology Ghent University Belgium 3 RIKEN Institute of Physicaland ChemicalResearch Saitama Japan Keywords frontalaffinity chromatography high-mannose-type glycans Jacalin-related lectin molecular evolution sugar-binding specificity Correspondence J. Hirabayashi Lectin Application and Analysis Team Research Center for Medical Glycoscience National institute of Advanced Industrial science and Technology AIST Tsukuba Central2 1-1-1 Umezono Tsukuba Ibaraki 305-8568 Japan Fax 81 29 861 3125 Tel 81 29 861 3124 E-mail jun-hirabayashi@ Received 17 October 2007 revised 21 December 2007 accepted 9 January 2008 doi The Jacalin-related lectin JRL family comprises galactose-binding-type gJRLs and mannose-binding-type mJRLs lectins. Although the documented occurrence of gJRLs is confined to the family Moraceae mJRLs are widespread in the plant kingdom. A detailed comparison of sugar-binding specificity was made by frontal affinity chromatography to corroborate the structure-function relationships of the extended mJRL subfamily. Eight mJRLs covering a broad taxonomic range were used Artocarpin from Artocarpus integrifolia jackfruit Moraceae BanLec from Musa acuminata banana Musaceae Calsepa from Calystegia sepium hedge bindweed Convolvulaceae CCA from Castanea crenata Japanese chestnut Faga-ceae Conarva from Convolvulus arvensis bindweed Convolvulaceae CRLL from Cycas revoluta King Sago palm tree Cycadaceae Heltuba from Helianthus tuberosus