tailieunhanh - Báo cáo lâm nghiệp: " Nutritive stress and cytokinin status in Norway spruce seedlings (Picea abies L. Karst.)"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài:" Nutritive stress and cytokinin status in Norway spruce seedlings (Picea abies L. Karst.)" | Ann. For. Sci. 62 2005 449-453 449 INRA EDP Sciences 2005 DOI forest 2005041 Original article Nutritive stress and cytokinin status in Norway spruce seedlings Picea abies L. Karst. Klaus v. Schwartzenberg Heinz Hahn Biozentrum Klein Flottbek und Botanischer Garten University Hamburg Ohnhorststr. 18 22609 Hamburg Germany Received 29 March 2004 accepted 14 March 2005 Abstract - The content of cytokinins was analysed in shoots and roots from spruce seedlings Picea abies L. Karst. grown on various hydroculture media either with complete nutrient supply control medium or with deficiencies for some nutrients stress medium . The cytokinins zeatin riboside and isopentenyladenosine were determined by an indirect competitive enzyme-linked immunosorbent assay ELISA in HPLC-purified extracts. The shoots of spruce seedlings grown on stress medium plus aluminum ions Al3 mM showed approximately 5-fold higher concentration of zeatin riboside and isopentenyladenosine than the controls. When the auxin naphtylacetic acid 1 pM was added to the stress medium also higher levels of cytokinin ribosides were measured in the shoots. In the roots however cytokinin riboside levels did not increase significantly neither under Al3 nor auxin treatment. The model experiments with spruce seedlings grown under controlled conditions support previous monitoring data on declining forest stands and show that nutritive stress can significantly increase the cytokinin content in the shoot of Norway spruce. cytokinin forest decline nutrient deficiency Norway spruce Picea abies Résumé - Stress nutritionnel et teneur en cytokinines chez des semis d epicea. Le contenu en cytokinines des parties aériennes et racinaires de jeunes semis d épicéas Picea abies élevés en culture hydroponique sur différents milieux a été étudié. Il s agissait soit de milieux comportant tous les éléments nutritifs témoin soit de milieux déficients en certains nutriments stress . Le contenu en cytokinines zéatine riboside