tailieunhanh - Báo cáo khoa học: "Charting the proteome of Cryptosporidium parvum sporozoites using sequence similarity-based BLAST searching"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Charting the proteome of Cryptosporidium parvum sporozoites using sequence similarity-based BLAST searching | J. Vet. Sci. 2009 10 3 203-210 DOI JOURNAL OF Veterinary Science Charting the proteome of Cryptosporidium parvum sporozoites using sequence similarity-based BLAST searching . Siddiki t Jonathan M. Wastling Department of Preclinical Veterinary Sciences Faculty of Veterinary Science University of Liverpool Crown Street Liverpool L69 7ZJ UK Cryptosporidium C. spp. are important zoonotic parasites causing widespread diarrhoeal disease in man and animals. The recent release of the complete genome sequences for C. parvum and C. hominis has facilitated the comprehensive global proteome analysis of these opportunistic pathogens. The well-known approach for mass spectrometry MS based data analysis using the BLAST tool MS BLAST is a database search protocol for identifying unknown proteins by sequence similarity to homologous proteins using peptide sequences produced by mass spectrometry. We have used several complementary approaches to explore the global sporozoite proteome of C. parvum with available proteomic tools. To optimize the output of the MS data a sequence similarity-based MS BLAST strategy was employed for bioinformatic analysis. Most significantly almost all the constituents of glycolysis and several mitochondrion-related proteins were identified. In addition many hypothetical Cryptosporidium proteins were validated by the identification of their constituent peptides. The MS BLAST approach was found to be useful during the study and could provide valuable information towards a complete understanding of the unique biology of Cryptosporidium. Keywords Cryptosporidium LC-MS MS MS BLAST proteomics sporozoites Introduction Cryptosporidium C. spp. are members of the phylum Apicomplexa and found in human and animal populations worldwide. People from both developed and developing countries are vulnerable to these opportunistic protozoa. It has a predilection for epithelial cells in the digestive tracts Corresponding author Tel .