tailieunhanh - Báo cáo khoa học: " Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells | J. Vet. Sci. 2009 10 3 181-187 DOI JOURNAL OF Veterinary Science Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells Min-Soo Seo1 2 3 t Yun-Hyeok Jeong1 2 3 1 Jeung-Ran Park1 2 3 Sang-Bum Park1 2 3 Kyoung-Hwan Rho1 2 3 1 2 3 X7--1 2 3 1 2 3 1 2 3 1 2 3 Hyung-Sik Kim Kyung-Rok Yu Seung-Hee Lee Ji-Won Jung Yong-Soon Lee Kyung-Sun Kang1 2 3 Adult Stem Cell Research Center 2Laboratory of Stem Cell and Tumor Biology Department of Veterinary Public Health and 3BK 21 program for Veterinary Sciences College of Veterinery Medicine Seoul National University Seoul 151-742 Korea Human umbilical cord blood-derived mesenchymal stem cells MSCs are known to possess the potential for multiple differentiations abilities in vitro and in vivo. In canine system studying stem cell therapy is important but so far stem cells from canine were not identified and characterized. In this study we successfully isolated and characterized MSCs from the canine umbilical cord and its fetal blood. Canine MSCs cMSCs were grown in medium containing low glucose DMEM with 20 FBS. The cMSCs have stem cells expression patterns which are concerned with MSCs surface markers by fluorescence- activated cell sorter analysis. The cMSCs had multipotent abilities. In the neuronal differentiation study the cMSCs expressed the neuronal markers glial fibrillary acidic protein GFAP neuronal class III p tubulin Tuj-1 neurofilament M NF160 in the basal culture media. After neuronal differentiation the cMSCs expressed the neuronal markers Nestin GFAP Tuj-1 microtubule-associated protein 2 NF160. In the osteogenic chondrogenic differentiation studies cMSCs were stained with alizarin red and toluidine blue staining respectively. With osteogenic differentiation the cMSCs presented osteoblastic differentiation genes by RT-PCR. This finding also suggests that cMSCs might have the ability to differentiate multipotentially. It was concluded that isolated MSCs .