tailieunhanh - Báo cáo khoa học: "The role of Bcl-xL and nuclear factor-kB in the effect of taxol on the viability of dendritic cells"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: The role of Bcl-xL and nuclear factor-kB in the effect of taxol on the viability of dendritic cells | J. Vet. Sci. 2009 10 2 99-103 DOI JOURNAL OF Veterinary Science The role of Bcl-xL and nuclear factor-K B in the effect of taxol on the viability of dendritic cells Mi-Hyoung Kim1 Hong-Gu Joo1A Laboratory of Veterinary Pharmacology College of Veterinary Medicine Radiological Science Research Institute Jeju National University Jeju 690-756 Korea Taxol has been used effectively in cancer therapies. Our previous study demonstrated that taxol induced altered maturation and improved viability of dendritic cells DCs . However the effects of taxol on DC viability have not been fully elucidated. In the present study flow cytometric analyses revealed that taxol treatment significantly increased the number of viable DCs and the expression levels of a representative anti-apoptotic protein Bcl-xL. Furthermore mobilization of the p65 subunit of nuclear factor-K B NF-K B from the cytosol to the nucleus in DCs was observed by confocal microscopy. An inhibition assay using N-p-tosyl-1-phenylalanine chloromethyl ketone confirmed that NF-KB was intimately involved in the effects of taxol on DC viability. In addition we investigated the mechanisms of taxol enhancement of DC viability. Since taxol is a popular anticancer agent used in clinic this study may provide a rationale for the use of taxol in DC immunotherapy to treat cancer patients. Taken together these results confirm that taxol increases DC viability and this information may provide new insights for new clinical applications of both taxol and DCs. Keywords apoptosis dendritic cell NF-kB taxol viability Introduction Taxol is a well-known anticancer drug used for many types of cancers including breast ovarian and non-small cell lung cancers 4 22 . Taxol is purified from Taxus brevifolia and acts as a microtubule-targeting anticancer drug by hindering the depolymerization of microtubules within cancer cells 21 . The effects of taxol on a variety of immune cells have been studied extensively.